| Highlights of Core R & D at ACERT
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Recent Highlights
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Portal to Lipoxygenase Active Site Revealed by Paramagnetic Distance Geometry
Betty J. Gaffney, Miles D. Bradshaw, Stephen Frausto (Biological Science, Florida State University, Tallahassee FL)
Jack H. Freed and Peter Borbat (ACERT)
June 2012
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Conformational States of Glutamate Transporters from High-Sensitivity Pulse Dipolar ESR
Elka R. Georgieva, Peter P. Borbat, Chris Ginter , Olga Boudker, and Jack H. Freed (June 2012)
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Pulsed ESR dipolar spectroscopy for distance measurements in immobilized spin labeled proteins in liquid solution
Zhongyu Yang, Yangping Liu, Peter Borbat, Jay L. Zweier, Jack H. Freed and Wayne L. Hubbell (June 2012)
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Detecting Gas-Phase Free Radicals in Mainstream Cigarette Smoke
Long-Xi Yu, Boris G. Dzikovski, and Jack H. Freed (June 2012)
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Conformational Distributions and Hydrogen Bonding in Gel and Frozen Lipid Bilayers: A High Frequency Spin-Label ESR Study
Boris Dzikovski, Dmitriy Tipikin†, and Jack H. Freed (June 2012)
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Dynamic Exchange Between Conformers Studied by Two-Dimensional Fourier Transform Electron Spin Resonance
Zhichun Liang and Jack H. Freed (July, 2010)
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Diphthamide biosynthesis requires a SAM-dependent [4Fe-4S]-containing enzyme
Yang Zhang, Xuling Zhu, Andrew Torelli, Michael Lee, Boris Dzikovski, Rachel M. Koralewski, Eileen Wang, Jack H. Freed, Carsten Krebs, Steven E. Ealick, and Hening Lin (July, 2010)
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Enhanced Sub-micron Resolution ESR Microscopy Applied to Cancerous Mouse Leg Tissue
Chang S. Shin, Curt R. Dunnam, Peter P. Borbat, Boris Dzikovski, Gene Barth, Howard Halpern, Michael Shklyar, and Jack H. Freed (July, 2010)
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Precise Real-Time Pulse Programmer for Time-Resolved FT ESR Spectroscopy
Boris D. Naumov, Peter P. Borbat, and Jack H. Freed (July, 2010)
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Pulse Dipolar Spectroscopy: Long-distances from 5-pulse DEER
Peter P. Borbat and Jack H. Freed (July, 2010)
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Studying Spin-Label Orientations by Two-dimensional DQC ESR
Peter P. Borbat, and Jack H. Freed (July, 2010)
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Multifrequency Electron Spin Resonance Study of T4 Lysozyme Dynamics
Ziwei Zhang, Dmitriy S. Tipikin, Zhichun Liang, Jozef K. Moscicki, Keith A. Earle, Mark R. Fleissner, Wayne L. Hubbell, Jack H. Freed (July 2009)
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Chemotactic Receptor Interactions with Histidine Kinase CheA and the Coupling Protein CheW revealed by Pulsed Dipolar ESR Spectroscopy
Jaya Bhatnagar, Abiola M. Pollard, Peter P. Borbat, Jack H. Freed, Alexandrine M. Bilwes, and Brian R. Crane (July 2009)
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Scanned-probe detection of electron spin resonance from a nitroxide spin probe
Eric W. Moore, Sang Gap Lee, Steven A. Hickman, Sarah J. Wright, Lee E. Harrell, Peter P. Borbat, Jack H. Freed, and John A. Marohn (July 2009)
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Molecular-scale force measurement in a coiled-coil peptide by electron spin resonance
Stefano V. Gullà, Gaurav Sharma, Constantinos Mavroidis, Peter Borbat, Jack H. Freed, Harischandra Ghimire, Gary A. Lorigan, Kaushal Rege, David E. Budil (July 2009)
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Membrane-Bound Alpha-Synuclein Forms an Extended Helix: Long-Distance Pulsed ESR Measurements Using Vesicles, Bicelles, and Rod-Like Micelles
Elka R. Georgieva, Trudy F. Ramlall, Peter P. Borbat, Jack H. Freed, David Eliezer (July 2009)
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A Scissor Mechanism for Stimulation of SNARE-Mediated Membrane Fusion by Cholesterol
Jiansong Tong, Peter P. Borbat, Jack H. Freed, Yeon-Kyun Shin (July 2009)
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Aggregation of spin labeled molecules by Dipolar Pulse ESR
Peter P. Borbat, Boris Dzikovski, and Jack H. Freed (June 2008)
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Fusion-peptide-induced vesicle swelling is associated with membrane surface ordering
Mingtao Ge and Jack H. Freed (June 2008)
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Conformational Motion of the ABC Transporter MsbA Induced by ATP Hydrolysis
Peter P. Borbat, Jack H. Freed, Kavitha Surendhran, Marco Bortolus, Ping Zou, Hassane S. Mchaourab (June 2008)
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Electron spin resonance spectra of a spin labeled protein calculated from molecular dynamics simulations
D. Sezer, J. H. Freed, B. Roux (June 2008)
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Using Markov models to simulate electron spin resonance spectra from molecular dynamics trajectories
D. Sezer, J. H. Freed, B. Roux (June 2008)
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Tie-Line Determination by ESR for Liquid-Ordered and Liquid-Disordered Coexistence Regions in a Model Membrane
A. K. Smith and J. H. Freed (June 2007)
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Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR
J. Bhatnagar, J.H. Freed, and B. R. Crane (June 2007)
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Two-Dimensional ELDOR Using Full Sc- Fitting and Absorption Lineshapes
Y.-W. Chiang, J.H. Freed, A. J. Costa-Fihlo (June, 2007)
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Human and Rat Monoamine Oxidases are Dimeric in the Mitochondrial Outer Membrane
A. K. Upadhaya, P.P Borbat, J. Wang, J.H. Freed, D. E. Edmondson (June, 2007)
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Sensitivity Improvement of Broadband High Frequency ESR Spectrometer: Application to HIV-Protease
D.S. Tipikin, K.A. Earle, J.H. Freed, L. Galiano, G. Fanucci (June, 2007)
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Inter-helix distances in lysophospholipid micelle-bound alpha-synuclein from pulsed ESR measurements
P. P. Borbat, J. H. Freed, Trudy F. Ramlall, and David Eliezer (June, 2006)
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Protein Dynamics from NMR: The Slowly Relaxing Local Structure Model with Rhombic Local Potential
Eva Meirovitch, Antonino Polimeno, J. H. Freed (June, 2006)
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Collective Fluctuations in Membrane Vesicles Studied by 2D-ELDOR
Barbara Fresch, Giorgio Moro, J. H. Freed (June, 2006)
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Dynamics of Nitroxide Side-Chain in Spin-Labeled Proteins
Fabio Tombolato, Alberto Ferrarini, J. H. Freed (June, 2006)
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Dynamic Exchange Incorporated into the Slowly Relaxing Local Structure Model
Zhichung Liang and Jack H. Freed (June, 2006)
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MAXIMUM ENTROPY: A Complement to Tikhonov Regularization for Determination of Pair Distance Distributions by Pulsed ESR
Yun-Wei Chiang, Peter P. Borbat, and Jack H. Freed (June, 2005)
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95 GHz 2D-ELDOR of spin-labeled Peptides in Membranes
K. A. Earle, B. Dzikowski, W. Hofbauer, J. K. Moscicki, and J. H. Freed (June, 2005)
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Pulsed ESR Studies of Interaction Interfaces Between T. Maritima CheA and CheW
S.-Y. Park, B. R. Crane, P. P. Borbat, A. M. Bilwes, J. H. Freed (June, 2005)
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ESR Method for Determining Tie-lines in Coexisting Membrane Phases
J. Zhao, J. Wu, G. W. Feigenson, Y. Shimoyama, Y.-W. Chiang, J. H. Freed (June, 2005)
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Distance Distributions in Protein Folding as Studied by Pulsed ESR: Cytochrome C
Y.-W. Chiang, P. P. Borbat, J. H. Freed, V. Grigoryantz, C. P. Scholes (June, 2005)
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Pulsed ESR Microscopy of Biodegradable Microspheres
Aharon Blank, Curt Dunnam, Peter Borbat, Jack H. Freed, Naraharisetti Pavan Kumar, and Chi-Hwa Wang (June, 2005)
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2D-ELDOR Studies Show Phase Structure Changes in Plasma Membrane Vesicles From RBL Cells Upon Stimulation
Yun-Wei Chiang, Jack H. Freed, Barbara A. Baird, David Holowka, Antonio J. Costa-Filho (June, 2005)
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ESR studies of alpha-synuclein topology
D. Eliezer, P. P. Borbat, J. H. Freed (June, 2005)
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The determination of pair distance distributions by pulsed ESR using Tikhonov regularization
Yun-Wei Chiang, Peter P. Borbat, and Jack H. Freed (June, 2004)
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Multifrequency ESR Studies of the Complex Dynamics of Membranes
Y.Lou, J. Freed, M. Ge (June, 2004)
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3D ESR microscopy with micron-scale resolution
A. Blank, C. Dunnam, P. P. Borbat, J. H. Freed (June, 2004)
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HFHF Multifrequency ESR on Aligned Membranes
B. Dzikovski, S. Pachtchenko, W. Hofbauer, K. A. Earle, J. H. Freed, (June, 2004)
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Protein Dynamics Using Multi-Frequency ESR: T4 Lysozyme
Z. Liang, Y. Lou, J. H. Freed, L. Columbus, and W. L. Hubbell (June, 2004)
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Effect Of Cholesterol Enrichment On Endoplasmic Reticulum Membranes
F. Maxfield, L. Ciani, I. Tabas, J. H. Freed, M. Ge, (June, 2004)
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Coexisting Domains In The Plasma Membranes Of Live Cells By ESR
M. J. Swamy, L. Ciani, D. Holowka, B. Baird, M. Ge, J. H. Freed (June, 2004)
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ESR Structural Studies of Peptoid Helices by Double-Quantum-Coherence ESR: Short Distances
Fafarman, B. Yoo, K. Kirshenbaum, P.P. Borbat, J.H. Freed (June, 2004)
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Measurement of Large Distances in Biomolecules Using Double-Quantum-Filtered Refocused Electron-Spin-Echoes
Peter P. Borbat, Jack H. Freed, Jared H. Davis, Samuel E. Butcher, (June, 2004)
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2D-ELDOR of the Liquid Ordered Phase in Multilamellar Vesicle Membranes
A. J. Costa-Filho, Y. Shimoyama, J. H. Freed (June, 2003)
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High Resolution ESR Microscopy
A. Blank, C. Dunnam, P. P. Borbat, J. H. Freed (June, 2003)
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High Power Pulsed ESR at 95GHz
W. T. Hofbauer, K. A. Earle, C. R. Dunnam, J. H. Freed (June, 2003)
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Study of Membrane Proteins by Double-Quantum Coherence ESR: Gramicidin A
P. P. Borbat, B. Dzikovski, J. H. Freed (June, 2003)
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Lipid-Gramicidin A' Interactions: Dynamic Structure of Boundary Lipid by 2D-ELDOR
P. P. Borbat, R. Crepeau, Z. Liang, J. H. Freed, A. Costa-Filho (June, 2002)
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Protein Structure Using Double-Quantum Coherence ESR: T4 Lysozyme
P. Borbat, J. Freed, H. Mchaourab (June, 2002)
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New Methods for Analyzing Heteronuclear NMR Relaxation Data from Proteins
E. Meirovitch, Z.Liang, J. Freed, V. Tugarinov (June, 2002)
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Single-crystal Rotation Studies in High-Field/High Frequency ESR
S. K. Misra, K. A. Earle, J. H. Freed (June, 2002)
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