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National Biomedical Resource
for Advanced Electron Spin Resonance Spectroscopy

Our research is supported by grant 1R24GM146107 from the National Institute of General Medical Sciences (NIGMS), part of the National Institutes of Health.

  Key publications
Model and Cell Membrane Dynamics by ESR
Articles:

The SARS–CoV Fusion Peptide Forms an Extended Bipartite Fusion Platform that Perturbs Membrane Order in a Calcium–Dependent Manner
A. L. Lai, J. K. Millet, S. Daniel, J. H. Freed, and G. R. Whittaker.
J. Mol. Biol. 429, 3875-3892 (2017)
<doi:10.1016/j.jmb.2017.10.017>
PMID: 29056462      PMCID: PMC5705393
 

Structure-Function Studies Link Class II Viral Fusogens with the Ancestral Gamete Fusion Protein HAP2
J. F. Pinello, A. L. Lai, J. K. Millet, D. Cassidy-Hanley, J. H. Freed, and T. G. Clark.
Curr. Biol. 27, 651-660 (2017)
Supporting Information
<doi:10.1016/j.cub.2017.01.049>
PMID: 28238660      PMCID: PMC5393271
 

Interaction of Spin-Labeled Lipid Membranes with Transition Metal Ions
B. Dzikovski, V. Livshits, and J.H. Freed
J. Phys. Chem. B 119, 13330-13346 (2015)
Supporting Information
<doi:10.1021/acs.jpcb.5b08165>
PMID: 26490692      PMCID: PMC4762260
 

The Interaction between Influenza HA Fusion Peptide and Transmembrane Domain Affects Membrane Structure
A.L. Lai and J.H. Freed
Biophys. J. 109, 2523-2536 (2015)
<doi:10.1016/j.bpj.2015.10.044>
PMID: 26682811      PMCID: PMC4699882
 

HIV gp41 Fusion Peptide Increases Membrane Ordering in a Cholesterol-Dependent Fashion
A.L. Lai and J.H. Freed.
Biophys. J. 106, 172-181 (2014).
<doi:10.1016/j.bpj.2013.11.027>
PMID: 24411249      PMCID: PMC3907210
 

Spin Labels in the Gel Phase and Frozen Lipid Bilayers: Do They Truly Manifest a Polarity Gradient?
B. Dzikowski and J.H. Freed.
In Nitroxides-Theory, Experiment and Applications; A. Kokorin. Ed.
InTech: Rijeka, Croatia, 2012; pp. 167-190.
<doi:10.5772/2887>
PMID: [None - Book]      PMCID: [None - Book]
 

Dynamics and Ordering of Lipid Spin-Labels Along the Coexistence Curve of Two Membrane Phases: An ESR Study
A.K. Smith and J.H. Freed.
Chem. Phys. Lipids, 165, 348-361 (2012).
Supporting Information
<doi:10.1016/j.chemphyslip.2012.02.009>
PMID: 22586732      PMCID: PMC3526980
 

Conformational Distributions and Hydrogen Bonding in Gel and Frozen Lipid Bilayers: A High Frequency Spin-Label ESR Study
B. Dzikovski, D. Tipikin, and J. Freed
J. Phys. Chem. B, 116, 6694-6706 (2012).
Supporting Information
<doi:10.1021/jp211879s>
PMID: 22324811      PMCID: PMC3376253
 

Membrane Fluidity
B. Dzikovski and J.H. Freed.
In Encyc. Biophys.; G.C.K. Roberts, Ed.
Springer Verlag: Berlin, Germany, 2013; pp 1440-1446.
<doi:10.1007/978-3-642-16712-6_546>
PMID: [None - Book]      PMCID: [None - Book]
 

Molecular Motions
S.K. Misra and J.H. Freed.
In Multifrequency Electron Paramagnetic Resonance, S.K. Misra, Ed.
Wiley-VCH: New York, 2011; Chapter 11, pp. 497-544.
PMID: [None - Book]      PMCID: [None - Book]
 

2D-ELDOR Study of Heterogeneity and Domain Structure Changes in Plasma Membrane Vesicles upon Cross-Linking of Receptors
Y.-W. Chiang, A.J. Costa-Filho, B. Baird, and J.H. Freed.
J. Phys. Chem. B 115, 10462-10469 (2011).
Supporting Information
<doi:10.1021/jp2016243>
PMID: 21780815      PMCID: PMC3165081
 

Two Conserved Residues Are Important for Inducing Highly Ordered Membrane Domains by the Transmembrane Domain of Influenza Hemagglutinin
M. Ge and J.H. Freed.
Biophys. J. 100, 90-97 (2011).
Supporting Information
<doi:10.1016/j.bpj.2010.11.014>
PMID: 21190660      PMCID: PMC3010018
 

Channel and Nonchannel Forms of Spin-Labeled Gramicidin in Membranes and Their Equilibria
B.G. Dzikovski, P.P. Borbat, and J.H. Freed.
J. Phys. Chem. B 115, 176-185 (2011).
Supporting Information
<doi:10.1021/jp108105k>
PMID: 21142163      PMCID: PMC3076037
 

Fusion Peptide from Influenza Hemagglutinin Increases Membrane Surface Order: An Electron-Spin Resonance Study
M. Ge and J.H. Freed.
Biophys. J. 96, 4925-4934 (2009).
Supporting Information
<doi:10.1016/j.bpj.2009.04.015>
PMID: 19527651      PMCID: PMC2712059
 

Determination of Tie-Line Fields for Coexisting Lipid Phases: An ESR Study
A.K. Smith and J.H. Freed.
J. Phys. Chem. B 113, 3957-3971 (2009).
<doi:10.1021/jp808412x>
PMID: 19673072      PMCID: PMC2772187
 

Membrane Fluidity
B. Dzikovski and J.H. Freed.
In Wiley Encyclopedia of Chemical Biology; T. P. Begley and B. A. Baird, Eds.
Wiley; Hoboken, NJ, USA, 2009; Chapter 2.
PMID: [None - Book]      PMCID: [None - Book]
 

Dynamic Molecular Structure and Phase Diagram of DPPC-Cholesterol Binary Mixtures: A 2D-ELDOR Study
Y.-W. Chiang, A.J. Costa-Filho, and J.H. Freed.
J. Phys. Chem. B 111, 11260-11270 (2007).
Supporting Information
<doi:10.1021/jp0732110>
PMID: 17760438     
 

High-Field ESR on Aligned Membranes: A Simple Method to Record Spectra from Different Membrane Orientations in the Magnetic Field
B. Dzikovski, K. Earle, S. Pachtchenko, J. Freed.
J. Magn. Reson. 179, 273-279 (2006).
<doi:10.1016/j.jmr.2005.12.015>
PMID: 16427793     
 

Coexisting Domains in the Plasma Membranes of Live Cells Characterized by ESR Spectroscopy
M.J. Swamy, L. Ciani, M. Ge, A.K. Smith, D. Holowka, B. Baird, and J.H. Freed.
Biophys. J. 90, 4452-4465 (2006).
Supporting Information
<doi:10.1529/biophysj.105.070839>
PMID: 16565045      PMCID: PMC1471862
 

New Method for Determining Tie-lines in Coexisting Membrane Phases using Spin-label ESR
Y.-W. Chiang, J. Zhao, J. Wu, Y. Shimoyama, J.H. Freed, and G.W. Feigenson.
Biochim. Biophys. Acta 1668, 99-105 (2005).
<doi:10.1016/j.bbamem.2004.11.010>
PMID: 15670735     
 

Dynamic Molecular Structure of DPPC-DLPC-Cholesterol Ternary Lipid System by Spin-Label Electron Spin Resonance
Y.-W. Chiang, Y. Shimoyama, G.W. Feigenson, and J.H. Freed.
Biophys. J. 87, 2483-2496 (2004).
<doi:10.1529/biophysj.104.044438>
PMID: 15454445      PMCID: PMC1304668
 

Spin-Labeled Gramicidin A: Channel Formation and Dissociation
B.G. Dzikovski, P.P. Borbat, and J.H. Freed.
Biophys. J. 87, 3504-3517 (2004).
<doi:10.1529/biophysj.104.044305>
PMID: 15326023      PMCID: PMC1304816
 

Enrichment of Endoplasmic Reticulum with Cholesterol Inhibits Sarcoplasmic-Endoplasmic Reticulum Calcium ATPase-2b Activity in Parallel with Increased Order of Membrane Lipids
Y. Li, M. Ge, L. Ciani, G. Kuriakose, E.J. Westover, M. Dura, D.F. Covey, J.H. Freed, F.R. Maxfield, J. Lytton, and I. Tabas.
J. Biol. Chem. 279, 37030-37039 (2004).
<doi:10.1074/jbc.M405195200>
PMID: 15215242     
 

Hydration, Structure, and Molecular Interactions in the Headgroup Region of Dioleoylphosphatidyl-Choline Bilayers: An Electron Spin Resonance Study
M. Ge and J.H. Freed.
Biophys. J. 85, 4023-40. (2003).
<doi:10.1016/S0006-3495(03)74816-4>
PMID: 14645091      PMCID: PMC1303703
 

Ordered and Disordered Phases Coexist in Plasma Membrane Vesicles of RBL-2H3 Mast Cells. An ESR Study
M. Ge, A. Gidwani, H.A. Brown, D. Holowka, B. Baird, and J.H. Freed.
Biophys. J. 85, 1278-1288 (2003).
<doi:10.1016/S0006-3495(03)74563-9>
PMID: 12885671      PMCID: PMC1303245
 

Lipid-Gramicidin Interactions: Dynamic Structure of the Boundary Lipid by 2D-ELDOR
A.J. Costa-Filho, R.H. Crepeau, P.P. Borbat, M. Ge, and J. H. Freed.
Biophys. J. 84, 3364-3378 (2003).
<doi:10.1016/S0006-3495(03)70060-5>
PMID: 12719265      PMCID: PMC1302896
 

A 2D-ELDOR Study of the Liquid Ordered Phase in Multilamellar Vesicle Membranes
A.J. Costa-Filho, Y. Shimoyama, and J.H. Freed.
Biophys. J. 84, 2619-2633, (2003).
<doi:10.1016/S0006-3495(03)75067-X>
PMID: 12668470      PMCID: PMC1302828
 

A Multifrequency ESR Study of the Complex Dynamics of Membranes
Y. Lou, M. Ge, and J.H. Freed.
J. Phys. Chem. B 105, 11053-11056 (2001).
<doi:10.1021/jp013226c>
 

ADP Ribosylation Factor 6 Binding to Phosphatidylinositol 4,5-Bisphosphate-Containing Vesicles Creates Defects in the Bilayer Structure: An Electron Spin Resonance Study
M. Ge, J.S. Cohen, H.A. Brown, and J.H. Freed.
Biophys. J. 81, 994-1005 (2001).
<doi:10.1016/S0006-3495(01)75757-8>
PMID: 11463641      PMCID: PMC1301569
 

Electron Spin Resonance Characterization of Liquid Ordered Phase of Detergent Resistant Membranes from RBL-2H3 Cells
M. Ge, K.A. Field, R. Aneja, D. Holowka, B. Baird, and J.H. Freed.
Biophys. J. 77, 925-933 (1999).
<doi:10.1016/S0006-3495(99)76943-2>
PMID: 10423437      PMCID: PMC1300383